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Project Overview

Project Code: MH 04

Project name:

Engineering resilient CAR-T against resistant cancer cells

TUM Department:

MH - Medicine

TUM Chair / Institute:

TranslaTUM, Hematology/Oncology Department

Research area:

Cellular Immunotherapy

Student background:

BiochemistryBiotechnologyMedicine

Further disciplines:

Participation also possible online only:

Planned project location:

TranslaTUM
Schmidts lab
Einstein Str. 25
81675 Munich

Project Supervisor - Contact Details


Title:

Dr.

Given name:

Andrea

Family name:

Schmidts

E-mail:

andrea.schmidts@tum.de

Phone:

+49 89 41409498

Additional Project Supervisor - Contact Details


Title:

Given name:

Family name:

E-mail:

Phone:

Additional Project Supervisor - Contact Details


Title:

Given name:

Family name:

E-mail:

Phone:

Project Description


Project description:

PROJECT
My lab develops engineered T cell therapies for cancer treatment. We focus on understanding and combating cancer cell resistance to CAR-T cell therapy. We have established a high-throughput CRISPR platform that allows for the functional characterization of tumor antigen variants, and we use the newest gene editing technologies to engineer CAR-T cells with improved resilience.

TUM PREP PARTICIPANTS TASKS
- production of CAR-T cells
- transduction of tumor cells with base editing libraries to install antigen variants in the endogenous locus
- set up positive selection screens using tumor cells that express antigen variants and CAR-T cells
- gDNA extraction form screen co-culture and library preparation for next-generation sequencing
- analysis of screening results

EXPECTED OUTCOMES
We expect to find tumor antigen variants that confer resistance to CAR-T cell therapy. Insights from this work will inform engineering efforts to make CAR-T cells less vulnerable to tumor resistance.

Working hours per week planned:

30-40

Prerequisites


Required study level minimum (at time of TUM PREP project start):

3 years of bachelor studies completed

Subject related:

Other:

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