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Project Overview

Project Code: NAT 19

Project name:

Peptide imaging using LD-REIMS

TUM Department:

NAT - Chemistry

TUM Chair / Institute:

Professorship for Analytical Chemistry

Research area:

Analytical Chemistry

Student background:

BiochemistryChemistry

Further disciplines:

Participation also possible online only:

Planned project location:

Department of Biosciences

Project Supervisor - Contact Details


Title:

Prof. Dr.

Given name:

Nicole

Family name:

Strittmatter

E-mail:

nicole.strittmatter@tum.de

Phone:

08928913321

Additional Project Supervisor - Contact Details


Title:

Msc

Given name:

Jiahao

Family name:

Li

E-mail:

jiahao.li@tum.de

Phone:

Additional Project Supervisor - Contact Details


Title:

Given name:

Family name:

E-mail:

Phone:

Project Description


Project description:

This project focuses on developing and optimising methods for drug detection and imaging LD-REIMS, a novel ambient ionisation technique. The experiments will be conducted on an enclosed mid-IR, nanosecond laser system by deploying an AP-IR-MALDI approach.
REIMS is an ambient mass spectrometry technique. When combined with nanosecond lasers, it offers the potential for high-resolution imaging, although its current applications are largely confined to lipidomics. Our goal is to broaden its chemical scope by integrating an AP-MALDI approach (or even reactive matrix apprach), with a particular focus on identifying matrices compatible with nanosecond IR lasers. However, systematic studies on matrix selection for this technique have not been performed yet.
Detection of drugs is essential to understand drug delivery, metabolism and resistance mechanisms, specifically in oncology settings.
To develop reliable workflows for peptide imaging, we will begin with a panel of small molecule chemotherapeutics that serve as standards for systematic method optimization. Once this workflow has been established, we aim to adapt it to high resolution spatial imaging of drug metabolism in tissues.

Experimental Techniques
 Laser Desorption/Ionization and REIMS Mass Spectrometry (LDI-REIMS)
 Preparation and sectioning of biological tissue samples
 Preparation and deposition of candidate matrices on samples
 Data acquisition, processing, and imaging analysis of mass spectrometry results

Possible Tasks for the Research Assistant
 Prepare tissue samples spiked with drugs
 Conduct imaging experiments using the LDI-REIMS system
 Test and compare different matrices under controlled conditions
 Collect, process, and interpret mass spectrometry data
 Summarize findings and contribute to method optimization protocols

Working hours per week planned:

35

Prerequisites


Required study level minimum (at time of TUM PREP project start):

3 years of bachelor studies completed

Subject related:

Experience with mass spectrometry or laser systems would be desirable

Other:

  • Keine Stichwörter