MH 18-Liver regeneration

Liver regeneration

MH - Medicine

Department of Surgery

Molecular biology, mouse experiments, in vitro experiments

BiochemistryBiologyMedicine

TUM Klinikum, Ismaninger Str. 22

Liver diseases play a major and growing role in global healthcare. In our hepatobiliary research group, we therefore investigate various aspects of carcinogenesis, fibrosis, and regeneration of the liver. To this end, we can draw on a wide range of in vitro experiments, mouse models, and clinical data and studies.

The organ's ability to regenerate after chronic damage or extensive resection poses an enormous challenge. This ability is determined by a complex interaction between the liver's own and foreign immune and non-immune cells.
The gut microbiome has a significant impact on various regenerative processes. In terms of the gut-liver axis, mouse experiments have demonstrated the influence of the microbiome on liver regeneration. Short-chain fatty acids (SCFAs) produced by certain bacterial species are relevant in this context. Dysbiosis of the microbiome has therefore been associated with reduced liver regeneration. At the same time, it has been shown that SCFA production can be influenced by manipulating the microbiome.

A current project is therefore investigating the influence of the gut microbiome on liver regeneration. To this end, a mouse experiment was initiated to experimentally modify the mouse microbiome and analyze the liver's ability to regenerate. In addition, a workflow for collecting data and samples from patients undergoing liver surgery is to be established in a clinical setting.

Students tasks:
- In vitro experiments with mouse and patient cells (e.g. PCR, cell culture, Western blot)
- Processing of human samples (e.g. collecting, freezing, cell isolation)
- Data base work

38

3 years of bachelor studies completed

Experience with molecular biology methods, e.g. PCR, ELISA and Western blot.