Anti-cFos (rabbit) | 1:2000 | (Abcam, ab222699, @-20°C)
For 26 slides, a total of 1.95 µL is needed. As we do stainings in 2 x 25 microscope slide batches, in one aliquot, ~4 µL of the antibody can be stored.
We want to stain ~300 microscope slides (6 mice x ~50 slides), therefore we need 6 aliquots.
6 aliquots x 4 µL = 24 µL
100 µL AB total volume - 24 µL = 76 µL remaining AB
For long-term storage, 4 x 19 µL aliquots with the remaining AB volume will be made.
Resulting aliquots:
6 aliquots x 4 µL
4 aliquots x 19 µL
Anti-Fos B (rabbit) | 1:1000 | (Abcam, ab184938, @-20°C)
For 26 slides, a total of 3.9 µL is needed. As we do stainings in 2 x 25 microscope slide batches, in one aliquot, ~8 µL of the antibody can be stored.
We want to stain ~300 microscope slides (6 mice x ~50 slides), therefore we need 6 aliquots.
6 aliquots x 8 µL = 48 µL
100 µL AB total volume - 48 µL = 52 µL remaining AB
For long-term storage, 4 x 13 µL aliquots with the remaining AB volume will be made.
Resulting aliquots:
6 aliquots x 8 µL
4 aliquots x 13 µL